CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage.
This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes.
CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect.
In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen): for discrimination of lymphomas from other tumors
Flow Cytometry: 1-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human CD53. Also reacts with deglycosylated molecule (molecular weight of the antigen is reduced by 15kD using endoglycosidase F).
Clone # USB：MEM-53
Isotype：IgG1
Calc Applications Abbrev：FC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Leukocytes of a patient suffering from a LGL-type leukemia.
