Transforming growth factor-beta (TGF-beta) family of proteins has potent hematopoietic regulatory properties ranging from effects on the growth and differentiation of primitive stem cells to the differentiated functions of mature cells.
Applications:Suitable for use in ELISA, Neutralization and Western Blot.
Other applications not tested.
Recommended Dilutions:Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line.
Tsang, M.
et al.
(1990) Lymphokine Res.
9:607.
The Neutralization Dose (ND50) is typically 0.25-1.25ug/mL in the presence of 1ng/mL recombinant human TGF‑ beta 1 and 7.5 ng/mL recombinant mouse IL‑4
Western Blot: 1ug/ml under non-reducing conditions onlyELISA: 2-8ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes bovine, chicken, mouse, and human TGF-beta. Recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3. No cross-reactivity was observed with recombinant LAP.
Clone # USB：10F520 (1D11)
Isotype：IgG1
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Bo Ch Hu Mo
Immunogen：Bovine bone-derived TGF-beta 1 and TGF-beta 2
