STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression.
After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA.
STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers.
STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation.
However, the both modifications are important for its maximal transcriptional activity.
On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human transcriptional factor STAT1 (91kD) activated by phosphorylation at Ser727 (intracellular antigen).
Clone # USB：PSM1
Isotype：IgG1
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：STAT1 peptide sequence 721-733, phosphorylated at Ser727.
