83-5957-76　Indoleamine 2,3-dioxygenase (Indoleamine-pyrrole 2,3-dioxygenase, IDO, INDO, Indoleamine 2,3-dioxygenase 1, IDO1, IDO-1) 100ug　I7545-06B

特徴

• Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines.
• IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma.
• IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response.
• IDO may be involved in the suppression of the immune response to tumors and blocking the IDO pathway may be a potential target for immunotherapy.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody.
• High temperature antigen retrieval is recommended
• Western Blot: Recognizes a 45kD band in IFN-gamma treated cell lines.
• Recognizes a slighty lower MW band in mouse IDO compared to its human counterpart
• Optimal dilutions to be determined by the researcher.
• Positive Control:IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
• Storage and Stability:May be stored at 4℃.
• For long-term storage, aliquot and store at 4℃.
• Do not freeze.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Further dilutions can be made in assay buffer.