CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets; surface expression indicates lysosomal secretion.
CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release; this phenomenon has been also reported for basophils.
CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI.
Both surface and cytoplasmic reactions are reported.
CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:10-1:25.
10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes the bovine homologue of the cell surface glycoprotein CD63.
