B7-DC is also called programmed death ligand 2 (PDL2).
It has recently been clustered as CD273.
This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines.
CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor.
CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.
C2549-21C1 has been reported to be useful for blocking PD-1 mediated interactions.
Applications:Suitable for use in Flow Cytometry and Blocking.
Though not tested, Immunohistochemistry, Immunoprecipitation and Western Blot have been reported in literature.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: ≤ 0.25ug labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography. Endotoxin: ≤0.01EU/ug (LAL)
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity：Recognizes mouse CD273. Blocks the binding of CD273 (B7-DC) to its’ receptor.
