83-5957-53　SIRP alpha (Signal Regulatory Protein alpha, SIRPA, Signal Regulatory Protein alpha Type 1, Signal Regulatory Protein alpha 1) (PE) 100Tests　S1013-86ZB3

特徴

• SIRP alpha is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells.
• SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways.
• Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2.
• These then act as regulators of cellular function, through dephosphorylation of specific substrates.
• Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
• Hybridoma:X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
• Storage and Stability:Store product at 4℃ before and after reconstitution.
• DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4℃.
• Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product centrifuge the original vial prior to removing the cap.