CD16 (FcgammaRIII) is a 50-65kD glycoprotein serving as a low affinity IgG receptor.
Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B.
FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells.
It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity.
Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit.
Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.
FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Applications:Suitable for use in Flow Cytometry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 3-12ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in TBS, pH 8.0, 0.09% sodium azide.
Specificity：Recognizes human CD16. Species Crossreactivity: monkey
Clone # USB：LNK16
Isotype：IgG1
Calc Applications Abbrev：FC IP
Calc Crossreactivity：Hu Mk
Immunogen：Normal human peripheral blood granulocytes.
