Desmosomes are adhesive intercellular junctions that act as cell surface attachment sites for intermediate filaments and play a role in providing strength to many tissues.
In epithelial cells, the cytoplasmic side of desmosomes, the ‘desmosomal plaque’, represents a specific attachment structure for the anchorage of intermediate filaments (IF) of the cytokeratin type.
Plakophilin-1 protein is one of several proteins that make up the desmosomal plaque, including plakoglobin and desmoplakin 1 and 2.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (frozen): Undiluted-1:50 Immunohistochemistry (paraffin): Undiluted-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5mL
抗体種類：Mab
宿主：mouse
Source Antibody：bovine
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes plakophilin 1 in diverse stratified epithelia, in complex epithelia (e.g. urothelium of the bladder and tracheal epithelium) and in glandular duct epithelia; squamous cell carcinoma are positive, whereas all adenocarcinoma tested so far are negative. Reactivity on human cultured cell lines: HaCaT, A-431, BPH-1 (derived from non-neoplastic prostatic tissue). Species Crossreactivity: human, bovine, porcine, mouse and rat
Clone # USB：PP1-5C2
Isotype：IgG1
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Plakophilin 1 ("band 6 polypeptide" of bovine muzzle epidermis)
