83-5957-34　CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (Biotin) 100ug　C2255-05A

特徴

• CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes.
• It is also present on 80% of thymocytes and at a lower level on monocytes.
• It is involved in recognition of antigen presented along with MHC class II by APCs.
• It serves as receptor for HIV.
• Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens.
• It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
• CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation.
• Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR).
• Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators.
• Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.
• Applications:Suitable for use in Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 1ug/ml; positive control: peripheral blood, HPB T cell lineOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.