The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation.
It is regarded as a specific probe for the cells of neuronal origin as well as for the tumors originating from these cells.
The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry.
Immunohistochemistry (paraffin sections):10ug/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue
Immunocytochemistry: with Neuro2a mouse neuroblastoma cell line
Flow Cytometry: 1-4ug/ml.
Intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin. Species Crossreactivity: canine, mouse, rat and porcine
Clone # USB：TU-20
Isotype：IgG1
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
