The betaIII isoform of tubulin is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation.
It is regarded as a specific probe for the cells of neuronal origin as well as for the tumors originating from these cells.
The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Applications:Suitable for use in Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-3ug/ml using intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes C-terminal peptide sequence ESESQGPK (aa441-448) of neuron-specific human betaIII-tubulin. Species Crossreactivity: canine, mouse, rat and porcine.
Clone # USB：TU-20
Isotype：IgG1
Calc Applications Abbrev：FC IC
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
