B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members.
Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication.
B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare.
The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages.
It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells.
B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses.
B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 5ul labels 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human CD276. Species Crossreactivity: African Green, Cynomolgus, Rhesus
