83-5957-14　Microphthalmia (Mi) 100ug　M3891-01C

特徴

• MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development.
• Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans.
• There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus.
• Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
• MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
• Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development.
• The mutation of Mi causes Waardenburg Syndrome type II in humans.
• In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.
• These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation.
• Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3′ half of Mi including most of the b-HLH-ZIP region.
• Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts.
• Applications:Suitable for use in Electrophoretic mobility shift assay, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: formalin-fixed, paraffin-embedded sectionsImmunoprecipitation: 2ug/mg of protein lysateWestern Blot: 1:500 detected Mi on 10ug of Mouse Brain lysates
• Optimal dilutions to be determined by the researcher.
• Control:501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.