DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation.
DNA-PKcs must bind DNA ends to become active.
DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins.
Phosphorylated DNA-PKcs is upregulated after DNA damage.
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Recognizes phosphorylated human DNA-PKcs (Thr2609)
Clone # USB：10B1
Isotype：IgG1,k
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic modified phospho-peptide corresponding to DNA-PKcs at Thr2609
