83-5956-90　Cytokeratin 18 (K18, CK18, CYK18, KRT18) 100ug　C9097-19N

特徴

• Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity.
• They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40kD and 68kD.
• The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Cytokeratin 18 belongs to type I family (acidic cytokeratins).
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 10ug/ml using human colon, skinFlow Cytometry: 1-4g/ml.
• Intracellular staining
• Optimal dilutions to be determined by the researcher.
• Positive Controls:Immunocytochemistry MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line
• ELISA: MCF-7 human breast adenocarcinoma cell line
• Matched Pairs:Detection: C9097-19NCapture: K0199-21A
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.