CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells.
CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation.
It is also expressed on neutrophils, monocytes and various cell lines.
Applications:Suitable for use in Blocking/Neutralization, Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4.
The Neutralization Dose (ND50) is typically 2.5-12ug/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha
Flow Cytometry: 0.25ug/10e6 cellsImmunohistochemistry (Paraffin): 8-25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human CXCR4. Species Crossreactivity: feline. Does not crossreact with Rat.
