Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry.
Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division.
Cdk1 also contributes to the control of DNA replication.
Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 10ug/mlWestern Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid is PBS. pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human Cdc2. Species Crossreactivity: bovine and monkey. Does not crossreact with mouse, rat, Drosophila melanogaster or Xenopus laevis.
Clone # USB：8J944 (POH-1)
Isotype：IgG2a
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Bo Hu Mk
Immunogen：Bacterially expressed full-length human Cdk1 protein
