83-5956-81　BUB1 (Bub1, Bub-1, BublL, Budding uninhibited by benzimidazoles 1-like) 100ug　B2790-01A

特徴

• The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes.
• This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint.
• Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1ug/ml on tissue culture cells.
• Fix with 3.5% PBS-buffered paraformaldehyde for 7 minutes.
• Permeabilization method is 0.2% Triton-X-100 after fixation with blocking buffer is 10mM Tris, pH 7.5 with 150mM sodium chloride with 0.1% BSA
• Immunoprecipitation: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.