Ca2+ plays a critical role in intracellular signaling.
Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX) in the plasma membrane.
NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+ and K+ gradient across the membrane.
In most cells, 3 Na+ are exchanged for 1 Ca2+.
In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2).
Applications:Suitable for use in Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Frozen, paraffin): 1:500 Western Blot: 1:1000.
Detects a band of ~140kD.
Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase
Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Ascites
Purity：Ascites
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes all known (currently four) isoforms of human plasma membrane PMCA ATPase. Recognizes an epitope between aa724-783 of human APTase, Ca. Species Crossreactivity: rat, mouse, sheep, canine, monkey, rabbit, feline, chicken and amphibian
Clone # USB：5F10
Isotype：IgG2a
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Ca Ch Fe Hu Mk Mo Rb Rt Sh
Immunogen：Purified human erythrocyte calcium ATPase.
