CD16 (FcgammaRIII) is a 50-65kD glycoprotein serving as a low affinity IgG receptor.
Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B.
FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells.
It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity.
Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit.
Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.
FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Applications:Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5-8ug/ml using PBL (peripheral blood lymphocytes)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Biotin
Specificity：Recognizes the epitope on human CD16 antigen that resides in proximity to FG loop (probably BC or C’E loop). Reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158. Does not react with CD16a present on NK cells in many subjects.
