CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g.
granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells.
There are several structural relatives of Lewis x, e.g.
sialyl-Lewis x or sulphated Lewis x.
Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction.
This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells.
Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins.
CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Applications:Suitable for use in Flow Cytometry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 1-4ug/mlOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in TBS, pH 8.0, 15mM sodium azide. Labeled with Biotin.
Specificity：Recognizes human CD15. Does not react with porcine, sheep or bovine
