CD14 is a 55kD GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor.
It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form.
CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein.
The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles.
CD14 affects allergic, inflammatory and infectious processes.
Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: non-reducing conditionsFlow Cytometry: 4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human CD14. Species Crossreactivity: non-human primates. In human, the epitope recognized is located between amino acids 57-64.
Clone # USB：MEM-18
Isotype：IgG1
Calc Applications Abbrev：E FC IP WB
Calc Crossreactivity：Hu
Immunogen：A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
