Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane.
The two have closely related structures, with 37% sequence homology (2).
They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes.
Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes.
A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes.
A minor pathway involves transport via the plasma membrane (3).
Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine ba based motif (YXXI) (1).
If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3).
This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1).
They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4)
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence (IC): 1:1000Western Blot: 1:1000, 1ug/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat LAMP1 at ~120kD. Species Crossreactivity: human, mouse and hamster
