The antibody should be incubated for 60 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mL
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes an internal, highly conserved amino acid sequence (VQEALD & VQAALD) found in both MDR1 and MDR3 isoforms of P-glycoprotein. The specific binding was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragment). Cross reacts with a 200kD protein that migrates in the same position as myosin and has also been reported to detect the 185kD c-erbB2 protein (Liu, et al., 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression. Species Crossreactivity: mouse, rat, canine and monkey
Clone # USB：6D562 (C219)
Isotype：IgG1,k
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Mk Mo Rt
Immunogen：SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
