Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV).
Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants.
These are identified based on serological reactivity of the epitopes on their outer membrane.
Intracellularly chlamydia replicates within a vacuole.
Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles.
This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell.
Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate.
H1830-71F detects Chlamydial HSP60 (GroEL).
It reacts with the short peptide of 6 amino acids from the carboxyl terminal region of HSP60.
HSP60 facilitates the correct folding of imported proteins and may prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:
Western Blot: 2ug/ml detected Chlamydial HSP60 in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Chlamydial HSP60, targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Clone # USB：A57-B9
Isotype：IgG1,k
Calc Applications Abbrev：E IC IF WB
Immunogen：Recombinant protein corresponding to Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
