Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation.
D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1.
Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase.
Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation.
Cyclin D1 destabilization participates in G1/S phase arrest.
Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (frozen and paraffin): 5-10ug/mlFlow Cytometry: 3-12ug/ml.
Intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human Cyclin D1at ~36kD. Species Crossreactivity: monkey, mouse and rat. Does not react with feline or sheep
Clone # USB：DCS-6
Isotype：IgG2a
Calc Applications Abbrev：FC IC IHC IP WB
Calc Crossreactivity：Ca Hu Mk Mo Rt
Immunogen：Full-length recombinant protein corresponding to aa1-295 from human cyclin D1.
