CD51/CD61 (integrin alpha5beta3), also known as osteoclast functional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor.
Expression of this antigen increases with melanoma progression.
In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 10ul labels 10e6 cells in 100ulOptimal dilution to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Puriffied
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes an extracellular epitope on the human CD51 and CD61 molecules (alpha V and beta 3 integrins). The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes. Species Crossreactivity: chicken. Does not crossreact with porcine, rabbit or bovine
Clone # USB：23C6
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Ch Hu
Immunogen：Cell suspension containing osteoclasts from osteoclastomas
