The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
Applications:Suitable for use in Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100Optimal working dilutions to be determined by researcher.
Hybridoma:SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from tissue culture supernatant.
Form：Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the V5 epitope tag. Detects recombinant proteins tagged with the epitope, some of which include transmembrane and secreted proteins. Usually, a 14aa tag is added to the recombinant proteins, although a smaller epitope of 9aa (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.
