CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro.
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development.
CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression.
CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling.
CD100 is expressed on resting and PHA stimulated T cells.
The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
Applications:Suitable for use in Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 5ul labels 10e6 cells in 100ul
Optimal working dilutions to be determined by researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human CD100. Species Crossreactivity: Cynomolgus monkey, Rhesus monkey
