CD34 is a highly glycosylated hematopoietic progenitor antigen.
Two isoforms of CD34 have been reported to be generated by alternative splicing.
This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis.
CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells.
CD34 is phosphorylated on serine residues by PKC.
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: ≤1ug labels 10e6 cells in 100ul.
Immunohistochemistry (frozen): 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
抗体種類:Mab
宿主:rat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Specificity:Recognizes mouse CD34. Does not stain bone marrow cells like some other mouse CD34 antibodies