The CD8 T cell coreceptor (monomer ~32-34kD) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells.
Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function.
Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts.
Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Highly Purified
Purity：Purified by size-exclusion chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human CD8. Species Crossreactivity: monkey
