Aggrecan is also known as aggregating chondroitin sulphate proteoglycan.
Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight.
It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
A1059-53C is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments.
The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences.
This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide
Specificity：Recognizes human Aggrecan, a neo-epitope on polypeptides with N-terminal FFGVG Sequences. By Western Blot, specifically detects G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase and matrilysin, but does not detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Species Crossreactivity: bovine, mouse, porcine and rat. Does not react with guinea pig or equine
Clone # USB：3H383 (AF-28)
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to FFGVGGEEDC from human Aggrecan, conjugated to KLH. Epitope: N-terminus FFGVG neoepitopes
