Plakophilin-1 (UniProt: Q13835; also known as Band 6 protein, B6P) is encoded by the PKP1 gene (Gene ID: 5317) in human.
Plakophilins are proteins that are involved in linking adherins to cytoskeletal intermediate filaments.
They contain numerous armadillo (ARM) repeats and localize to cell desmosomes and nuclei.
Plakophilin-1 may be involved in molecular recruitment and stabilization during desmosome formation.
It is also shown to be important for tooth development and regulates ZO-1 distribution in the dental epithelium.
Two isoforms of Plankophilin-1 have been reported that are produced by alternative splicing.
Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia and isoform 2 is widely expressed.
Plakophilin-1 is shown to be highly expressed in teeth and skin, and was upregulated during tooth development.
It is reported to be involved in the regulation of Wnt signaling via its ARM repeat domains in a manner similar to beta-catenin and Wnt activation induces its nuclear translocation.
Mutations n PKP1 gene are shown to cause ectodermal dysplasia-skin fragility syndrome that is characterized by cutaneous fragility and congenital ectodermal dysplasia affecting skin, hair and nails.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml detected Plakophilin 1 in 10ug of PC3 cell lysate
Optimal dilutions to be determined by the researcher.
Positive Control:MCF-7 cell lysates
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Planophilin-1 and targets an epitope with in the first 27 amino acids from the N-terminal region.
Clone # USB：3G250 (10B2)
Isotype：IgG1
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：MBP-conjugated recombinant fragment corresponding to the first 235 amino acids from the N-terminal region of human Plakophilin-1. Species sequence homology: mouse and rat
