The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration.
The b-subunit (95kD) possesses a single transmembrane domain, whereas the a-subunit (135kD) is completely extracellular.
Applications:Suitable for use in Immunoprecipitation, Tyrosine Kinase Assay and Inhibition.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation (Use Protein G): 2ug/mg protein lysateTyrosine Kinase Assay: Antibody-mediated capture on microtiter plates Inhibits Insulin Binding: 10nM (1,2)Optimal dilutions to be determined by the researcher.
Positive Control:IM-9 lymphocyte cells.
Placenta.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human Insulin receptor, alpha subunit. Inhibits insulin binding (~80%) and mimics insulin action. Species Crossreactivity: Weakly reacts with bovine, porcine and sheep. Does not react with rabbit, mouse or rat.
Clone # USB：3F220
Isotype：IgG2a,k
Calc Applications Abbrev：IP N
Calc Crossreactivity：Bo Hu Po Sh
Immunogen：IM-9 lymphocytes followed by purified insulin receptor. MW of Antigen: 135kD. Epitope: aa469-592 (exon 7/8). Cellular Localization: Cell membrane.
