Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in K562 (human chronic myelogenous leukemia) cell line.
c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution.
c-Abl protein is distributed in both nucleus and cytoplasm of cells.
In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to Bcr gene on chromosome 22.
The molecular consequence of translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase.
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunoprecipitation: 2ug/mg protein lysate.
Use Protein G
Western Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 12-24ug/ml for 20 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cells or placenta
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in PBS, pH 7.4. Also supplied with BSA and sodium azide. See C0013-02A.
Specificity：Recognizes human c-Abl p120 and chimeric Bcr/Abl proteins. Species Crossreactivity: mouse
Clone # USB：2Q217 (8E9)
Isotype：IgG1
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to c-Abl. Cellular Localization: Cytoplasmic and nuclear. Epitope: SH2 domain. MW of Antigen: 120kD.
