83-5955-71　Progesterone Receptor, phosphorylated (Ser294) 100ul　P9007-16A

特徴

• There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005).
• The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms).
• In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003).
• Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) .
• Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser-190 and Ser-294 with different kinetics.
• These phosphorylation events may differentially affect the transcriptional activity of the receptor.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.