Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells.
It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3).
A family of ligands known as the angiopoietins binds to Tie2.
Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5).
Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7).
Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: 1:50Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Tie2 in various endothelial cell lines. Does not cross-react with related proteins. Species Crossreactivity: bovine
Clone # USB：4i64 (AB33)
Isotype：IgG1
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Hu
Immunogen：Recombinant protein fragment corresponding to the extracellular domain of human Tie2.
