Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament.
These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues.
Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis .
These can be divided into acid (pI <5.7) and basic (pI >6.0) subfamilies.
The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD.
The basic keratins have molecular weights of 65-67, 64, 59, 58, 56, and 52kD.
Members of the acidic and basic subfamilies are found together in pairs.
The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state: The 56.5/65-67kD pair is present in keratinized (differentiated) epidermis.
The 55/64kD pair is characteristic of normal (corneal-type) epithelial differentiation.
The 51/59kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue.
The 51/58kD pair is a keratinocyte marker; the pair is present in almost all stratified epithelia irrespective of the state of cellular stratification.
The 48/56kD pair is characteristic of hyperproliferative (de-differentiated) keratinocytes.
The 45/52kD pair and to a lesser extent, the 46/54kD pair are characteristic of simple epithelia.
The 40kD keratin is present in most epithelia except adult epidermis.
The stringent, but broad, specificity of pooled AE1/AE3 antibody has made this preparation very useful as a general stain for normal and neoplastic cells of epithelial origin.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 0.5-2ug/ml (unfixed frozen, formalin-fixed and paraffin embedded sections).
Note: For proper staining on paraffin embedded tissues, trypsin or pepsin digestion is required.
(Trypsin: 1mg/ml for 10min at 37℃.
Or Pepsin: 1mg/ml for 5 min at 37℃)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by sodium sulfate precipitation.
Form：Supplied as a liquid in borate buffered saline, pH 8.0, 0.09% sodium azide.
Specificity：Recognizes the 56.5, 50, 50', 48 and 40kD keratins of the acidic subfamily. Recognizes all members of the basic subfamily (65, 67, 64, 59.59, 56, 52). Species Crossreactivity: human, bovine, chicken, monkey, mouse, rabbit and rat
