CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer.
CD8a is a 34 kD protein that belongs to the immunoglobulin family.
The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells.
CD8 expression on mature T cells is non-overlapping with CD4.
The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells.
CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells.
CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Applications:Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation.
Not recommended for immunohistochemistry of formalin-fixed paraffin sections.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 0.25ug labels 1x10e6 cells in 100ulImmunohistochemistry: Acetone fixed frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
抗体種類：Mab
宿主：rat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
