83-5955-47　Insulin Receptor 200ug　I7661-22B

特徴

• The insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD).
• The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site.
• The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain.
• The biological effects of insulin are mediated primarily through the insulin receptor.
• The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity.
• Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation.
• These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.
• Applications:Suitable for use in Immunoprecipitation, Blocking and Immunoblot.
• Other applications not tested.
• Recommended Dilution:Immunoprecipitation: using radiolabelled human insulin receptor (hINSR) (Soos et al, 1986)Blocking: has been shown to cause >80% inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and to block receptor activation
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.