CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, ~60% of NK active cells and on a subset of T cells.
Applications:Suitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT.
Note: No special pretreatment is rquired for staining of formalin-fixed, paraffin embedded tissues
Optimal dilutions to be determined by the researcher.
Positive Control:Lymph node or tonsil
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2411-26.
Specificity:Recognizes human CD57 at 110kD. Marks a subset of lymphocytes known as natural killer (NK) cells. Also reacts with a variety of cell types in non-lymphoid tissues. Stains neuroendocrine cells and their tumors.
Clone # USB:0.N.152
Isotype:IgM,k
Calc Applications Abbrev:FC IHC
Calc Crossreactivity:Hu
Immunogen:Membrane extracts of the HSB-2 T lymphoblastoid cell line. Cellular Localization: Cell membrane