Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which stimulates the growth and survivability of certain B cells and T cells.
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils.
IL-4 elicits many different biological responses, but has two dominant functions.
The first is regulating differentiation of naive CD4+ T cell to the TH2 type.
TH2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled bu TH1 cells.
The second is regulating IgE and IgG1 production by B cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (100ul/well): 0.5-1ug/ml Detects ≥0.2ng recombinant human IL-4/well using I8426-03 as detection antibody
Western Blot: 0.2-0.5ug/ml.
When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-4 is 0.25-0.5ng/lane, under reducing conditions or 1-2ng/lane, under non-reducing conditions
Optimal dilutions to be determined by the researcher.
Hybridoma:ICRF1 cells with spleen cells from Balb/c mice.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from ascites.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes human Interleukin-4.
Clone # USB：8.PP.3
Isotype：IgG1,k
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin-4, expressed in E. coli.
