CellMosaic's Phenyldiazirine sxLink™ （T2A14） is a proprietary photo-crosslinking reagent developed at CellMosaic® for studying biomolecular interactions.This reagent combines the highly efficient carbene-generating phenyldiazirine group with a cleavable disulfide crosslinking group via a super hydrophilic AqueaTether™ （AqT®） based linker.
These structural features make it highly advantageous to use this reagent over traditional photo-crosslinking reagents.1.
Trifluoromethyl phenyldiazirine （TFMP） group： TFMP photolyzes around 360 nm, at which photodamage to biomolecules is minimized.
The generated carbene inserts C–H bonds into the neighboring biomolecular partner within picoseconds.
Because the electron-withdrawing trifluoromethyl group confers stability on the intermediate diazo-isomer, no side products are detected under normal labeling conditions.2.
Reversible disulfide for chemical crosslinking of any biomolecule containing a free thiol.3.
Hydrophilic AqT® linkers： TFMP group is highly hydrophobic, biomolecules labeled with a TFMP using a traditional ethylene and ethylene glycol-type linker tend to aggregate and destabilize the labeled protein.
T2A14 AqT® linker is made by linking two threitols together, greatly increasing the hydrophilicity of sxLink™ and its water solubility （2.2 mg/mL saturated solution）.
The molecule is also more biocompatible with decreased non-specific hydrophobic interactions with other biomolecules, allowing high loading of phenyldiazirine groups.The product is sold as a lyophilized powder in either 1 vial of 250 μg （Cat# CM81401-250UG） or 4 vials of 250 μg （Cat# CM81401-4x250UG）.
One vial of dissolution buffer is included in each configuration.
