HIC HPLC analysis is a useful tool to separate bioconjugates labeled with very hydrophobic small molecules based on their hydrophobicity.
The hydrophobic interaction of the bioconjugates and the column media is affected by the presence of salts or organic solvent in the running buffer.CellMosaic® has designed this high quality HIC buffer set for your convenient analysis of bioconjugates, such as antibody-drug conjugate （ADC） and protein or peptide-drug conjugate following the preparation of the bioconjugates using CellMosaic's Personalized Conjugation Kit™ （PerKit™）.This HIC buffer set comes with two buffers and instruction for usage.
Buffer A has high salt and Buffer B contains no salt.
A high salt concentration aids the interaction of the bioconjugates with the column media.
Usually, a decreasing salt gradient is used to elute samples from the column.
As such, the lowest degree of labeled bioconjugate （low hydrophobicity） is eluted first and the most labeled bioconjugates elute last.
