Stabilizing buffer for long-term storage of antibody-drug conjugates （ADCs）, protein-drug conjugates, or any antibody/protein that has been modified with very hydrophobic small molecules.Antibody-drug conjugates （ADCs） are new types of therapeutics currently being developed by many pharmaceutical and biotech companies.
An ADC generally involves linking a very hydrophobic chemical compound to an antibody.
ADCs linked through classical linkers tend to aggregate and precipitate out of solution over time.
The general guideline at CellMosaic® is to prepare ADCs using our conjugation kits immediately before use.
Some ADCs may be stored at 4 °C for a few weeks or months.
Freezer storage of ADCs is generally not recommended due to the possibility of accelerated aggregation or precipitation during the freezing process.CellMosaic® has developed proprietary ADC stabilizing buffers that can be used for long-term storage of ADCs.
The buffers contain stabilizers to prevent the hydrophobic drugs from interacting with one another and keep the ADCs in solution when stored below freezing conditions.
Stabilizers also help preserve the structure of the ADCs during lyophilization.
After lyophilization, ADCs can be dissolved in deionized water without changing their properties.
Lyophilized ADCs can also be shipped or stored at RT temporarily.
Furthermore, stabilizing buffers are biocompatible and will not interfere with any in vitro or in vivo studies.
The buffer does not contain preservative, protease inhibitors, reducing agents, metal chelators such as EDTA, or other carrier proteins.
If needed, all buffer components can be removed by dialysis or desalting before use in downstream assays.Note： the degree of stabilizing effects is antibody- and drug-specific.
CellMosaic® recommends checking the ADCs periodically by HPLC to ensure its shelf life in ADC stabilizing buffer.
