概要：Large-scale transfection of mammalian cells has emerged as an indispensable technology within the scientific community.
It allows for the efficient production of milligram-to-gram quantities of r-proteins within a short timeframe, just a few days after the cloning of cDNAs into the suitable expression vector.
Among the various cell lines available, the HEK293 cell line stably expressing the Epstein-Barr virus nuclear antigen-1 (HEK293-EBNA1, or 293E) stands out as the most widely utilized cell line for large-scale transfection experiments.
One of the critical advantages of utilizing HEK293-EBNA1 cells is their compatibility with expression vectors carrying the Epstein-Barr virus origin of replication, oriP, such as the pTT vector.
This compatibility leads to a significant threefold improvement in the yield of r-proteins compared to using a non-oriP vector.
Leveraging this potential, researchers can now achieve higher yields of their desired r-proteins, bringing them closer to their research and development goals.
