概要：The RWPE-1 cell line, originating from the peripheral zone of a histologically normal prostate from a 54-year-old Caucasian male, epitomizes a key resource for biomedical research, particularly in the realms of infectious and sexually transmitted diseases.
This epithelial cell line, characterized by its adherence growth properties and epithelial morphology, was established through the transfection of human papilloma virus 18 (HPV-18) into the cells.
Notably, the RWPE-1 cells, catalogued as ATCC CRL-11609, maintain a predominantly diploid karyotype at passage 32, with variations including 45, X,-Y and 51, XY populations.
The cell line expresses critical markers such as Kallikrein 3 (KLK3), also known as prostate-specific antigen (PSA), alongside cytokeratins 8 and 18, and tumor suppressors p53 and pRB, with androgen exposure inducing PSA secretion.
This is particularly evident in 3-dimensional Matrigel cultures where RWPE-1 cells form acinar structures and secrete PSA into the lumen.
