概要：HT-1080 cells are an epithelial cell line derived from the connective tissue of a 35-year-old male patient with Fibrosarcoma in 1972.
These cells have been extensively utilized in biomedical research due to their diverse applications and unique characteristics.
With their origin in Fibrosarcoma, HT-1080 cells offer researchers a valuable resource to study and understand the behavior and progression of this disease.
These cells carry an IDH1 mutation and an activated N-ras oncogene, making them particularly suitable for investigating the effects of these genetic alterations.
HT-1080 cells exhibit morphological features characteristic of epithelial cells derived from connective tissue.
They are adherent cells, requiring a suitable substrate for growth.
The applications of HT-1080 cells are wide-ranging.
They have proven a valuable tool in 3D cell culture, providing a physiologically relevant model for studying cell behaviour within a three-dimensional context.
Additionally, HT-1080 cells have been extensively used in bioproduction processes.
HT-1080 cells have a doubling time of approximately 26 to 30 hours and have a karyotype with a modal number of 46 chromosomes, although some variability has been observed.
Roughly 40% of the cells exhibit rearranged karyotypes, including an additional E-group chromosome and a probable loss of a group C chromosome, possibly chromosome 11.
One notable characteristic of HT-1080 cells is their tumorigenic potential.
They have been shown to form tumours in immunosuppressed mice, making them an appropriate model for studying tumour development and metastasis.
The presence of an activated N-ras oncogene further contributes to their tumorigenic properties.
Moreover, HT-1080 cells have been widely used to study tumour growth and metastasis as a xenograft model and intramuscularly implantation in nude mice results in primary tumours and lung metastases.
Tail-vein injection of HT-1080 cells in nude mice leads to the development of lung metastases.
Subcutaneous implantation in immunosuppressed or nude mice results in subcutaneous tumour formation.
Additionally, HT-1080 cells have been found to metastasize to the lung, liver, brain, and mammary gland when injected into appropriate animal models.
