概要：The A375 cell line was isolated from the skin of a 54-year-old female patient with malignant melanoma.
The cells have a diameter of roughly 12 microns, which is relatively small compared to other cell lines.
A375 cells have been widely used in various applications such as 3D cell culture, high-throughput screening, toxicology, and immuno-oncology.
The growth properties of A375 cells are adherent with an epithelial morphology.
A375 cells are highly tumorigenic and produce rapidly growing amelanotic melanomas in anti-thymocyte serum-treated NIH Swiss mice.
In immunosuppressed mice, A375 cells have also been found to be tumorigenic.
A375 cells have a hypotriploid karyotype with a modal number of 62 chromosomes.
Nine marker chromosomes are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.
The doubling time for A375 cells is around 6-12 hours, which means these cells can divide and proliferate quickly under favorable conditions.
A375 melanoma cells harbor the BRAFV600E mutation and are highly sensitive to MEK inhibition.
Treatment of A375 melanoma cells with vemurafenib has enhanced the induction of MHC Class I and Class II molecules by interferon gamma and IFN alpha 2b.
In conclusion, A375 cells are a highly useful cell line widely used in various applications such as 3D cell culture, high-throughput screening, toxicology, and immuno-oncology.
